A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR-2

Erated from eleven cDNA targets quantified using five reaction formulations (see Figure 2 and additional file for additional details). Average linear regression correlation coefficients (r). Average E. Average ΔE. Note that ΔE is dependent on the optics of the assay and are thus not directly comparable across different formulations and gain settings. Average Fderived from the average of Fvalues encompassed by the LRE window (see Figure 4 for details). : QuantiTect, : DyNAmo; : FullVelocity, : SYBR Green I, : standard deviation, : the number of runs, : not applicable (only one run conducted), : not determined.<p><b>Copyright information:</b></p><p>Taken from "A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR"</p><p>http://www.biomedcentral.com/1472-6750/8/47</p><p>BMC Biotechnology 2008;8():47-47.</p><p>Published online 8 May 2008</p><p>PMCID:PMC2397388.</p><p>