Results of RT-PCR of AGS (a), AT-1 (b), vit-D bp (c) and Tis21 (d) genes. A result of GAPDH gene was shown as a control (e). Products of RT-PCR with cycles indicated in each panel using mRNA from KO and WT mice were separated in an acrylamide gel, and EtBr-stained bands were obtained, showing almost equivalent levels (AGS, AT-1, vit-D bp as well as GAPDH) or reduced expression levels in KO (Tis21).<p><b>Copyright information:</b></p><p>Taken from "Disruption of GM2/GD2 synthase gene resulted in overt expression of 9--acetyl GD3 irrespective of Tis21"</p><p></p><p>Journal of Neurochemistry 2008;105(3):1057-1066.</p><p>Published online Jan 2008</p><p>PMCID:PMC2408653.</p><p>© 2008 The Authors Journal compilation © 2008 International Society ...
<p>Real-time PCR analyses were carried out on 3-mm slices of kidneys from <i>Mrp2<sup>−/−</sup></i> ...
<p>(A) Semi-quantitative RT-PCR analysis confirmed that the identified mRNA species by DNA microarra...
<p>(A) Gene expression determined by RT-PCR or quantitative RT-PCR analysis was examined in Neuro2A ...
Astrocytes were cultured with or without GD3 for 24 h, and expression of the gangliosides were analy...
<p>Mouse AAT and NE mRNAs were measured by RT-PCR and factored by simultaneously obtained GAPDH prod...
<p>RNA samples were obtained from either WT or <i>Mecp2</i><sup>-/y</sup> mice at two different ages...
<p>A) Semi-quantitative Real-Time PCR revealed significantly higher <i>Aqp9</i> mRNA levels in the m...
<p>The expression of genes related to mitochondria was analyzed using quantitative real-time PCR. Th...
<p>Quantitative real-time PCR analysis was carried out with LVs from WT (n = 10), control (n = 15) a...
<p>The log<sub>2</sub> transformation of the fold-change of the relative mRNA expression levels in t...
<p>Relative expression levels are normalized to Glyceraldehyde 3-phosphate dehydrogenase (GADPH). St...
<p>Liver samples 1 and 2 were from control mice; samples 3, 4, and 5 were from mice exposed to hyper...
(a) TLC of brain gangliosides from the WT and KO mice prepared via alkaline treatment. (b) TLC of br...
<p>(a) Illustration of anti-hCD63-coated magnetic beads used to isolate hCD63-positive ELMs from sal...
<p>mRNA was prepared from the left ventricles of WT or SOCS3-CKO mice after reperfusion and subjecte...
<p>Real-time PCR analyses were carried out on 3-mm slices of kidneys from <i>Mrp2<sup>−/−</sup></i> ...
<p>(A) Semi-quantitative RT-PCR analysis confirmed that the identified mRNA species by DNA microarra...
<p>(A) Gene expression determined by RT-PCR or quantitative RT-PCR analysis was examined in Neuro2A ...
Astrocytes were cultured with or without GD3 for 24 h, and expression of the gangliosides were analy...
<p>Mouse AAT and NE mRNAs were measured by RT-PCR and factored by simultaneously obtained GAPDH prod...
<p>RNA samples were obtained from either WT or <i>Mecp2</i><sup>-/y</sup> mice at two different ages...
<p>A) Semi-quantitative Real-Time PCR revealed significantly higher <i>Aqp9</i> mRNA levels in the m...
<p>The expression of genes related to mitochondria was analyzed using quantitative real-time PCR. Th...
<p>Quantitative real-time PCR analysis was carried out with LVs from WT (n = 10), control (n = 15) a...
<p>The log<sub>2</sub> transformation of the fold-change of the relative mRNA expression levels in t...
<p>Relative expression levels are normalized to Glyceraldehyde 3-phosphate dehydrogenase (GADPH). St...
<p>Liver samples 1 and 2 were from control mice; samples 3, 4, and 5 were from mice exposed to hyper...
(a) TLC of brain gangliosides from the WT and KO mice prepared via alkaline treatment. (b) TLC of br...
<p>(a) Illustration of anti-hCD63-coated magnetic beads used to isolate hCD63-positive ELMs from sal...
<p>mRNA was prepared from the left ventricles of WT or SOCS3-CKO mice after reperfusion and subjecte...
<p>Real-time PCR analyses were carried out on 3-mm slices of kidneys from <i>Mrp2<sup>−/−</sup></i> ...
<p>(A) Semi-quantitative RT-PCR analysis confirmed that the identified mRNA species by DNA microarra...
<p>(A) Gene expression determined by RT-PCR or quantitative RT-PCR analysis was examined in Neuro2A ...