Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by ELISpot-analysis in contact dermatitis patients-0

Or PHA (1 μg/ml) were expressed as Index values. Index is expressed by the ratio between the number of spot forming cells (IFN-γ and IL-10 producing PBMC) upon stimulation with metal salts and those present in the absence of stimuli (spontaneous cytokine production). The mean values of Index values ± SD were obtained for the 4 different groups of 10 subjects according to their reactivity to metal salts. Cytokine producing cells in control triplicate wells with medium alone was the baseline value (Index = 1). Panel : patch test positive to Ni (group 1); : patch test positive to Pd (group 2); : patch test positive to Ni and Pd (group 3); : negative patch test (group 4). The statistical analysis between IFN-g and IL-10 producing cells upon stimulation with Ni or Pd between patients belonging to group C and A or B was performed using the Mann-Whitney test. The highly significant differences (P < 0.0001), found between group C and B, are indicated by symbols (*, **, °, °°).<p><b>Copyright information:</b></p><p>Taken from "Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by ELISpot-analysis in contact dermatitis patients"</p><p>http://www.biomedcentral.com/1471-2172/9/19</p><p>BMC Immunology 2008;9():19-19.</p><p>Published online 15 May 2008</p><p>PMCID:PMC2409297.</p><p>