Distinct and stage specific nuclear factors regulate the expression of falcipains, cysteine proteases-4

of double stranded nucleotide probes used in EMSA. Astericks denote those probes that formed complexes with nuclear extracts. (B) Electrophoretic mobility shift assay. End labeled double-stranded oligonucleotides were incubated with 2–5 μg of nuclear proteins isolated from asynchronous parasite culture. For competition experiments, a 100 fold excess of the respective unlabelled double-stranded oligonucleotides were used. Arrows indicate the positions of DNA-protein complexes.<p><b>Copyright information:</b></p><p>Taken from "Distinct and stage specific nuclear factors regulate the expression of falcipains, cysteine proteases"</p><p>http://www.biomedcentral.com/1471-2199/9/47</p><p>BMC Molecular Biology 2008;9():47-47.</p><p>Published online 14 May 2008</p><p>PMCID:PMC2409366.</p><p>