(A) Nuclear extracts were prepared from isolated Kupffer cells 1 hr after addition of leptin (100 nM), and AP-1 DNA binding activity was analyzed by EMSA

lane 1; probe alone, lane 2; control, lane 3; leptin, lane 4; IL-6 (positive control), lane 5; X250 competitor. (B) Kupffer cells were incubated with leptin for 3–6 hr, and TGF-beta 1 mRNA was detected by ribonuclease protection assay.<p><b>Copyright information:</b></p><p>Taken from "Expression of leptin receptors in hepatic sinusoidal cells"</p><p>http://www.comparative-hepatology.com/content/3/S1/S12</p><p>Comparative Hepatology 2004;3(Suppl 1):S12-S12.</p><p>Published online 14 Jan 2004</p><p>PMCID:PMC2410235.</p><p>