(A) Cells isolated from the spleen and mesenteric LNs of mice were sorted into eight distinct populations based on their expression of GFP, CD3, TCR-β, TCR-δ, CD4, and CD25 (Fig

S1), and gene expression was assessed using real-time PCR. Ct values were normalized to the mean Ct of five housekeeping genes. Data are the mean of two or three independent experiments. (B) Foxp3 RORγt T cells express IL-10. Cells isolated from LNs of mice were restimulated in vitro with PMA/ionomycin for 5 h and subjected to intracellular staining for GFP, IL-17, Foxp3, and IL-10 or an isotype control. Numbers indicate percent cells in quadrants. Results are representative of at least three individual experiments.<p><b>Copyright information:</b></p><p>Taken from "In vivo equilibrium of proinflammatory IL-17 and regulatory IL-10 Foxp3 RORγt T cells"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1381-1393.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413035.</p><p>