Co-expression of eYFP-XIE-T or eYFP-XIK-T with various fluorescent organelle markers in tobacco epidermal cells

eYFP-XIE-T (magenta a, e, i, m) was co-expressed with fluorescent markers for Golgi bodies (ST-CFP, b), peroxisomes (CFP-SKL, f), mitochondria (β ATPase signal peptide-GFP, j), and the ER (GFP-HDEL, n). The merged images indicate that eYFP-XIE-T is closely associated, but does not solely decorate the periphery of Golgi bodies (c), peroxisomes (g), mitochondria (k), or the ER (o). Merged images of cells co-expressing eYFP-XIK-T (magenta) with fluorescent markers (green) for Golgi bodies (d), peroxisomes (h), mitochondria (l), and ER (p) also show a close association of eYFP-XIK-T with these organelles. Both eYFP-XIE-T and eYFP-XIK-T are present in large and small puncta. eYFP-XIE-T is in more numerous small puncta than eYFP-XIK-T, whereas eYFP-XIK-T is more diffuse throughout the cytosol than eYFP-XIE-T (compare c with d). Scale bar 5 μm.<p><b>Copyright information:</b></p><p>Taken from "Truncated myosin XI tail fusions inhibit peroxisome, Golgi, and mitochondrial movement in tobacco leaf epidermal cells: a genetic tool for the next generation"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2499-2512.</p><p>Published online 23 May 2008</p><p>PMCID:PMC2423659.</p><p>