Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters-1

Hylcellulose in optiMEM. At 6 days p.i., the cells were fixed in methanol and immunostained with ferret polyclonal antibody directed to hMPV followed by anti-ferret horse radish peroxidase-conjugated antibody. The immunostained plaques were treated with 3-amino-9-ethylcarbazole for visualization. B) Replicate cultures of Vero cells were inoculated with rhMPV or rhMPV/ΔM2-2 at MOI of 0.1 PFU/cell and incubated at 35°C. Supernatants and cells were harvested daily for 4 days. Titers were determined by plaque assay in Vero cells. The graph represents an average +/- SD titer of three independently performed experiments.<p><b>Copyright information:</b></p><p>Taken from "Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters"</p><p>http://www.virologyj.com/content/5/1/69</p><p>Virology Journal 2008;5():69-69.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2426676.</p><p>