Detection of autoantigens in experimental autoimmune uveoretinitis (EAU)

Shown is a two-dimensional gel image of murine retinal proteins stained with SYPRO Ruby. Approximately 2,000 spots were observed. Among the SYPRO Ruby stained protein spots, the 2D-WB positive spots either in control or EAU were randomly numbered. The numbers and the positions of the seven candidate autoantigens in EAU on a SYPRO Ruby stained gel are shown in panel . The spot numbers of the candidate autoantigens are common between panel , , and . Extracted murine retinal proteins were separated by two-dimensional electrophoresis, transferred onto nitrocellulose membranes. Western blotting was performed using sera from EAU or control mice. Representative membranes reacted with sera from complete Freund's adjuvant-treated control mice are shown in subpanel , and those reacted with sera from EAU mice are shown in subpanel . Each set of and subpanels shows the corresponding area. Arrowheads indicate the position of each candidate autoantigen on the EAU membranes. membranes.<p><b>Copyright information:</b></p><p>Taken from "Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens"</p><p></p><p>Molecular Vision 2008;14():1094-1104.</p><p>Published online 12 Jun 2008</p><p>PMCID:PMC2426731.</p><p>