Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model-1

NF. Every bar represents change in normalization accuracy when stepwise adding more endogenous controls according to ranking in Fig. 1. Data were analyzed for the three different time points 4 days (A), 7 days (B) and 21 days (C) after ACA. The use of the two most stable genes is in each case sufficient for an accurate normalization (cutoff 0.15 according to [19]). The higher V9/10 is due to the inclusion of a relative unstable gene, , and is in accordance with the average expression stability M.<p><b>Copyright information:</b></p><p>Taken from "Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model"</p><p>http://www.biomedcentral.com/1471-2199/9/53</p><p>BMC Molecular Biology 2008;9():53-53.</p><p>Published online 28 May 2008</p><p>PMCID:PMC2430208.</p><p>