Fura-2–loaded wild-type (black line) or (gray line) platelets were stimulated with 0

1 U/ml thrombin, 10 μM ADP, or 10 μg/ml CRP in the presence of extracellular 1 mM EGTA or 0.5 mM Ca, and [Ca] was monitored. Representative measurements (A) and maximal increase in intracellular Ca concentrations compared with baseline levels before stimulus (Δ[Ca ]) ± SD ( = 4 mice per group; B) are shown. (C) Impaired aggregation of platelets (gray lines) in response to CRP and collagen but not ADP and thrombin (recording time = 10 min). (D) Flow cytometric analysis of integrin αIIbβ3 activation (binding of JON/A-PE; left) and degranulation-dependent P-selectin exposure (right) in response to 0.1 U/ml thrombin, 10 μM ADP, 10 μg/ml CRP, and 1 μg/ml convulxin. Results are means ± SD ( = 6 mice per group). (E) platelets in whole blood fail to form stable thrombi when perfused over a collagen-coated (0.2 mg/ml) surface at a shear rate of 1,700 s. (top) Representative phase-contrast images. (bottom) Mean surface coverage (left) and relative platelet deposition as measured by the integrated fluorescent intensity per square millimeter (right) ± SD ( = 4 mice). ***, P < 0.001. Bar, 100 μm.<p><b>Copyright information:</b></p><p>Taken from "The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1583-1591.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442636.</p><p>