Reciprocal transplantation assay was performed by transplantation of BM (before mutation was induced) into lethally irradiated WT mice (A) and, reciprocally, WT BM into lethally irradiated mice (before mutation was induced; C)

deletion was induced 1 mo after transplantation by injecting the recipient mice with polyI:C every other day for a total of three injections. mice were used as respective controls in this experiment (B and D). On day 8 after the first polyI:C injection, recipient mice were killed for analysis. BM sections from the recipients showed that HSC/Ps failed to grow in the WT BM environment (A), whereas the BM microenvironment was able to support the growth of WT HSC/Ps (C). (E–H) Significant reduction of WBC counts and plt in peripheral blood, and nucleated cells in the BM and spleen in WT recipients that received BM. (E) Obvious increase in WBC counts in recipients that received WT BM. (I and J) Equal numbers of BM cells (CD45.2, before mutation was induced) were mixed with WT (CD45.1) BM cells and transplanted into lethally irradiated WT (CD45.1) recipients. 6 wk after transplantation, mutation was induced by injecting the recipient mice with polyI:C every other day for a total of three injections. BM cells were transplanted in parallel as a control. The contribution of the BM HSC/Ps to hematopoiesis in the recipient mice (shown as CD45.2 percentage in peripheral blood) was significantly lower than that of control BM HSC/Ps. Data shown in I and J were analyzed on day 15 after the first polyI:C injection. *, P < 0.05; **, P < 0.01.<p><b>Copyright information:</b></p><p>Taken from "TAK1 is required for the survival of hematopoietic cells and hepatocytes in mice"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1611-1619.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442639.</p><p>