(A and B) Western blot analysis of total PTEN in control (A) or IPF (B) fibroblasts cultured on monomeric or polymerized collagen in growth factor–replete media

One representative sample out of five control and five IPF cell lines examined is shown. (C and D) PTEN phosphatase assay showing the percent change in PTEN activity in control ( = 3; C, left) or IFP ( = 3; D, left) fibroblasts cultured on polymerized or monomeric collagen in growth factor–replete media compared with cells cultured under proliferation-prohibitive conditions. *, P < 0.001 for PTEN activity in control fibroblasts on polymerized collagen that was significantly higher at day 3 compared with monomeric collagen; P < 0.01 for PTEN activity in IPF fibroblasts on polymerized collagen that was significantly less on day 3 compared with monomeric collagen. Shown is PI3K activity in control (C, right) and IPF (D, right) fibroblasts cultured on monomeric or polymerized collagen in growth factor–replete media for 3 d. *, P < 0.005 for PI3K activity in control fibroblasts cultured on polymerized collagen that was significantly less at day 3 compared with monomeric collagen; or P < 0.05 for PI3K activity in IPF fibroblasts cultured on polymerized collagen that was significantly higher at day 3 compared with monomeric collagen. Error bars represent SEM. (E) PTEN protein levels in membrane and cytosolic fractions of cell lysates were measured by Western blot analysis. Data are representative of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Pathological integrin signaling enhances proliferation of primary lung fibroblasts from patients with idiopathic pulmonary fibrosis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1659-1672.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442643.</p><p>