(A) TSA breast cancer cells were subcutaneously injected in WT and Sema4D KO mice

Tumor volume was evaluated at the indicated days. As shown, tumor burden was dramatically less in Sema4D KO versus WT mice. Bars indicate mean tumor volume ± SEM. *, P < 0.0001 ( = 10 mice per group). (B and C) Macro- and micrometastases present in the lungs of mice bearing tumors of comparable size were evaluated as indicated in Materials and methods and scored. Error bars indicate mean ± SD. *, P < 0.0001 ( = 10 lungs). (D) Representative pictures of lungs and lung slices for WT and Sema4D KO mice. As shown, the number of metastases was significantly lower in Sema4D KO mice. Statistical differences between groups were determined by using the two-tailed Student's test. Bars: (whole mount) 0.5 cm; (micrograph) 0.2 mm.<p><b>Copyright information:</b></p><p>Taken from "Tumor angiogenesis and progression are enhanced by Sema4D produced by tumor-associated macrophages"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1673-1685.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442644.</p><p>