Wnt-5a mRNA and protein expression in Huh7 cells transfected with the HBx3′-30 or HBx3′-40 mutant, and in human clinical HCC and corresponding non-cancerous liver tissue samples

() RT–PCR. Wnt-5a mRNA was upregulated in HBx3′-30-transfected Huh7 cells but downregulated in HBx3′-40-transfected Huh7 cells, compared with cells expressing full-length HBx. Wnt-5a mRNA expression varies in human HCCs when compared with their corresponding non-cancerous liver tissues. () Real-time RT–PCR. No statistically significant difference in Wnt-5a mRNA expression was found between HCC and the corresponding non-cancerous liver tissues. () Western blotting. The expression of Wnt-5a protein was upregulated in HBx3′-30-transfected Huh7 cells, but was not significantly different in HBx3′-40-transfected Huh7 cells, compared with cells transfected with full-length HBx. () The protein was present at significantly lower levels in the tumor tissues than in the corresponding non-cancerous liver tissue ( < 0.01); N, non-tumor; T, HCC; Bars, mean; columns, standard deviation.<p><b>Copyright information:</b></p><p>Taken from "Mutations in the C-terminus of the X protein of hepatitis B virus regulate Wnt-5a expression in hepatoma Huh7 cells: cDNA microarray and proteomic analyses"</p><p></p><p>Carcinogenesis 2008;29(6):1207-1214.</p><p>Published online 13 May 2008</p><p>PMCID:PMC2443277.</p><p>