Roles of WRKY3 and WRKY4 Transcription Factors in Plant Responses to Pathogens-4

Nd mutants. Wild-type and mutant plants were sprayed with SA (1 mM). The leaves were harvested 4 hours after treatment for total RNA isolation. After separation on the gels and blotting to nylon membranes, the blots were probed with corresponding gene-specific DNA fragments. and expression in transgenic plants. RNA samples were prepared from leaves of 5-week-old wild-type (Col-0) and transgenic plants and probed with a - or -specific probe. Transgenic lines 3 and 8 and transgenic lines 7 and 13 contained a single T-DNA insertion in the genome and exhibited stable expression of their respective transgenes. Their F3 homozygous progeny plants were used in all the experiments in the study.<p><b>Copyright information:</b></p><p>Taken from "Roles of WRKY3 and WRKY4 Transcription Factors in Plant Responses to Pathogens"</p><p>http://www.biomedcentral.com/1471-2229/8/68</p><p>BMC Plant Biology 2008;8():68-68.</p><p>Published online 20 Jun 2008</p><p>PMCID:PMC2464603.</p><p>