Electrophoresis of polymerase chain reaction-restriction fragment length polymorphism products for detection of nucleotide alterations of and

DNA fragments with G at codon 141 () contained the Apa I restriction site, resulting in fragments of 304 bp+264 bp, while DNA fragments with T lacked the Apa I restriction site, resulting in a fragment of 568 bp. DNA fragments with A at codon 153 () contained the Hinf I restriction site, resulting in fragments of 335 bp+233 bp, while DNA fragments with G lacked the Hinf I restriction site, resulting in a fragment of 568 bp. Information for : M; 100 bp marker, Lane 1; HO (negative control), Lane 2; GG (positive control), Lane 3; TT (positive control), Lane 9 and 10; GG, Lane 5, 6, and 8; TG, Lane 4 and 7; TT. Information for : M; 100 bp marker, Lane 1; HO (negative control), Lane 2; AG (positive control) Lane 3; GG (positive control), Lane 5, 6, and 7; GG, Lane 4, 8, and 10; AG, Lane 9; AA.<p><b>Copyright information:</b></p><p>Taken from "Lysyl oxidase-like 1 gene polymorphisms in Japanese patients with primary open angle glaucoma and exfoliation syndrome"</p><p></p><p>Molecular Vision 2008;14():1303-1308.</p><p>Published online 14 Jul 2008</p><p>PMCID:PMC2464611.</p><p>