ATP-induced autophagy is associated with rapid killing of intracellular mycobacteria within human monocytes/macrophages-4

-II (16 kDa) by Western blot. Lane 2 represents untreated control THP-1 cells. B. ATP (3 mM) treated MDMs also express LC3-II (lane 2), which is absent in non-treated control cells (lane 1). C. THP1 cells treated with 3 mM ATP for 30 minutes cultured in calcium-free medium (lane 1) and calcium-replete medium (lane 2). LC3-II expression was observed in ATP-treated cells cultured in calcium-replete medium (lane 2) but was absent in cells cultured in calcium-free media (lane 1). D. THP1 cells were treated with different P2 agonists and antagonists. Lane 1 represents untreated control cells, while lanes 2 and 3 represent cells pre-treated with oATP (0.3 mM) and anti-P2Xantibody (3 μg/ml) for 2 hours and 1 hour respectively, prior to treatment with ATP (3 mM for 30 minutes). Lanes 4, 5 and 6 represent cells treated with ATP (3 mM), UTP (3 mM) and bzATP (3 mM) for 30 minutes. The presence of an LC3-II band, indicating cell autophagy, was observed only in lanes 4 and 6.<p><b>Copyright information:</b></p><p>Taken from "ATP-induced autophagy is associated with rapid killing of intracellular mycobacteria within human monocytes/macrophages"</p><p>http://www.biomedcentral.com/1471-2172/9/35</p><p>BMC Immunology 2008;9():35-35.</p><p>Published online 15 Jul 2008</p><p>PMCID:PMC2483257.</p><p>