Cigarette smoke worsens lung inflammation and impairs resolution of influenza infection in mice-7

Tokine in lung were determined by real time PCR (a-c only, right). BALFs of the 8 mice in each group were pooled and used in duplicate ELISAs. Clear boxes denote mice not infected with influenza and dark boxes denote mice infected with influenza. Legend: ■ = influenza alone mice, ▲ = smoke alone and - ◆ - = smoke and influenza mice. Tissue pieces of equivalent size from lungs of the 4 mice in each treatment group were pooled prior to RNA extraction, and the cDNA was used in triplicate reactions. Fold changes are expressed relative to mRNA levels in lungs of no treatment mice. Cytokines assayed were (a) TNF-α, (b) MIP-2, (c) GM-CSF, (d) IFN-γ and (e) MCP-1.<p><b>Copyright information:</b></p><p>Taken from "Cigarette smoke worsens lung inflammation and impairs resolution of influenza infection in mice"</p><p>http://respiratory-research.com/content/9/1/53</p><p>Respiratory Research 2008;9(1):53-53.</p><p>Published online 15 Jul 2008</p><p>PMCID:PMC2483272.</p><p>