Repulsion by Slit and Roundabout prevents Shotgun/E-cadherin–mediated cell adhesion during heart tube lumen formation-1

Robo (green). (A) A wild-type embryo showing the normal pattern of Slit. (B) embryo with one copy each of and ( GOF) in which Slit is no longer restricted to the CB apical domains. (C) Wild type pattern of Robo. (D) Robo is mislocalized to CB basal surfaces (arrow) in a GOF embryo. (E) EM of a wild-type embryo in XS showing the lumen (arrow) that forms between two CBs. (F) EM of a GOF embryo showing the two-lumen phenotype. Arrowheads indicate cell membranes. (G and H) Close up views of E and F. Arrowheads indicate the extracellular matrix. (I and J) XSs of embryos stained for Mef2, which labels CB and somatic muscle nuclei (brown). (I) The lumen is visible between CBs (arrowhead) in a wild-type embryo. (J) GOF embryo with two lumens (arrowheads). Bars: (A) 6 μm; (E) 2 μm; (G) 0.5 μm.<p><b>Copyright information:</b></p><p>Taken from "Repulsion by Slit and Roundabout prevents Shotgun/E-cadherin–mediated cell adhesion during heart tube lumen formation"</p><p></p><p>The Journal of Cell Biology 2008;182(2):241-248.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2483515.</p><p>