(A) β-galactosidase staining (representing expression) of whole-mounted hearts from embryos from E9

5 to 11.5. Arrows point to the AV canal or OFT at E11.5. Bars, 250 μm. (B) Sections through the AV canal and OFT of β-galactosidase–stained hearts from E9.5 to 11.5. Bars, 100 μm. (C) Immunofluorescence staining for Slug (red) and CD31 (green) in E11.5 embryonic mouse hearts. Arrowheads point to cells coexpressing Slug and CD31. Bars, 25 μm. (D) In situ hybridization for and in a 65-d human embryonic heart. Arrows point to the mitral and tricuspid valves, arrowheads indicate the interatrial septum, and the asterisk marks the AV septum. A higher magnification image of the heart valve is shown in the right panel of each analysis. Bars, 1 mm.<p><b>Copyright information:</b></p><p>Taken from "Slug is a direct Notch target required for initiation of cardiac cushion cellularization"</p><p></p><p>The Journal of Cell Biology 2008;182(2):315-325.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2483533.</p><p>