(A) Schematic diagram of RT-PCR strategy for the identification of CEACAM1 long and short cytoplasmic domain splice variants

Binding sites for the forward and reverse primers and the expected size of amplified fragments are shown. The PCR amplified products were separated by electrophoresis on a 2.5% agarose gel. M, refers to 100 bp DNA ladder (NEB). (B) Histogram shows CEACAM1-S/CEACAM1-L (S:L) ratio quantified with NIH ImageJ program.<p><b>Copyright information:</b></p><p>Taken from "Altered splicing of CEACAM1 in breast cancer: Identification of regulatory sequences that control splicing of CEACAM1 into long or short cytoplasmic domain isoforms"</p><p>http://www.molecular-cancer.com/content/7/1/46</p><p>Molecular Cancer 2008;7():46-46.</p><p>Published online 28 May 2008</p><p>PMCID:PMC2490704.</p><p>