Importin alpha binding and nuclear localization of PARP-2 is dependent on lysine 36, which is located within a predicted classical NLS-3

Blot using a monoclonal anti-HA antibody. 50 μg of whole cell extracts were used, endogenous PARP-1 levels served as loading control. HEK293T cells were transfected with HA-tagged wild type (wt) PARP-2 or with the PARP-2 mutants K36R and K37R. HA-tagged proteins were detected by immunofluorescence as described for Figure 2A. Representative images are presented.<p><b>Copyright information:</b></p><p>Taken from "Importin alpha binding and nuclear localization of PARP-2 is dependent on lysine 36, which is located within a predicted classical NLS"</p><p>http://www.biomedcentral.com/1471-2121/9/39</p><p>BMC Cell Biology 2008;9():39-39.</p><p>Published online 21 Jul 2008</p><p>PMCID:PMC2496901.</p><p>