(A) Acrosome reaction of sperm from the epididymis and vas deferens was induced by calcium ionophore A23187

Acrosome-intact and -reacted sperm were subjected to western blot anaylsis with the anti-Shsp1/Mm.87328 antibody. AI, acrosome-intact sperm; AR, acrosome-reacted sperm. (B) Sperm from the epididymis and vas deferens were treated with 1% Triton X-100 and urea with different concentrations (2, 3, 4 and 6 M). Soluble and insoluble fractions after centrifugation of the treated sperm were subjected to western blot anaylsis with the anti-Sfap1/Mm.386907 and anti-Sfap2/Mm.157049 antibodies. Sup, supernatant after centrifugation; Pt, pellet after centrifugation.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p>