(A and B) Frames (A) and kymograph (B) from a time-lapse recording showing single molecules of Eg5-GFP (green) moving directionally along a microtubule (red) in the presence of 70 mM Pipes

(C and D) Frames (C) and kymograph (D) from a time-lapse recording showing single molecules of Eg5-GFP (green) diffusing along a microtubule (red) in the presence of 70 mM Pipes plus 40 mM KCl. (E–H) MD calculated from Eg5-GFP motility in the presence of ATP (black) and ADP (red) in 70 mM Pipes plus 0, 20, 40, or 60 mM KCl. Fits represent MD = . (I–M) MSD calculated from Eg5-GFP motility in the presence of ATP (black) and ADP (red) and at the indicated ionic strengths. Fits represent MD = and MD 2 + offset for the ATP data and MD 2 + offset for the ADP data. All numerical results are listed in . (N) Histogram of the duration of binding events for 0 mM KCl and for 60 mM KCl added. Lines are single exponential fits (exp[−t/t]) to the data (0 mM: t = 34 ± 3, = 212; 60 mM: t = 16 ± 2, = 119). Error bars represent SD. Bars, 1 μm.<p><b>Copyright information:</b></p><p>Taken from "Microtubule cross-linking triggers the directional motility of kinesin-5"</p><p></p><p>The Journal of Cell Biology 2008;182(3):421-428.</p><p>Published online 11 Aug 2008</p><p>PMCID:PMC2500128.</p><p>