Use of cancer-specific yeast-secreted biotinylated recombinant antibodies for serum biomarker discovery-4

bound to case-pool serum. The enriched yeast-display scFv were labeled with anti-c-myc mAb and 25 μg/ml (A,B,E,F) or 100 μg/ml (C,D,G,H) of biotinylated case (A,C,E,G) or control (B,D,F,H) serum pools, before (A-D) or after depletion by magnetic sorting (E-H). Binding signals were detected as shown with the secondary antibody 488-alexa anti-mouse Ig (488 anti-mIg) and PE-labeled streptavidin (SA-PE).<p><b>Copyright information:</b></p><p>Taken from "Use of cancer-specific yeast-secreted biotinylated recombinant antibodies for serum biomarker discovery"</p><p>http://www.translational-medicine.com/content/6/1/41</p><p>Journal of Translational Medicine 2008;6():41-41.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2503970.</p><p>