(A) Arabidopsis, ecotype Landsberg and gene trap mutant line GT8359, uninfected, or infected with (TYMV) or (TCV), as indicated

(B) Transmission electron microscope image confirming the presence of viral particles (arrowed) in the leaves of a TYMV-infected plant. The length of the bar corresponds to 100 nm. The image was prepared by Jean Devonshire of the Rothamsted Centre for Bioimaging. (C) Western analyses showing immunodetection of AtGCN2, phosphorylated AteIF2α (AteIF2α-) or total AteIF2α in crude protein extracts from leaves of Arabidopsis, ecotype Landsberg or gene trap mutant line GT8359, as indicated. The leaves had been inoculated with TYMV or TCV as indicated. A positive control of extracts from herbicide IRL-treated seedlings and a negative control of leaves inoculated with water are included.<p><b>Copyright information:</b></p><p>Taken from "GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3131-3141.</p><p>Published online 4 Jul 2008</p><p>PMCID:PMC2504353.</p><p>