A comparative study of protein synthesis in systems: from the prokaryotic reconstituted to the eukaryotic extract-based-5

L systems. B. Different reporter proteins (indicated) were synthesized for 60 min. in the reactions containing S-methionine in the PURE system (lane 1), the PURE system supplemented with the fraction 17 (lane 2) and in the S30 system (lane 3). Aliquots of the reactions were run on a SDS-PAGE gel, followed by autoradiography. The arrows indicate the positions of the full-length proteins. C. A time course of synthesis of Fluc in different systems (indicated) and at three temperatures (37°C, filled ovals with solid line; 30°C, open ovals with solid line; 20°C, open ovals with dash line). The activities are averages of duplicate experiments and presented as the percentage of the relative light unit determined at 37°C and 60 min.<p><b>Copyright information:</b></p><p>Taken from "A comparative study of protein synthesis in systems: from the prokaryotic reconstituted to the eukaryotic extract-based"</p><p>http://www.biomedcentral.com/1472-6750/8/58</p><p>BMC Biotechnology 2008;8():58-58.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2507708.</p><p>