Select Overexpression of Homer1a in Dorsal Hippocampus Impairs Spatial Working Memory-1

Ng (TBP) induced LTP in hippocampal CA3-CA1 synapses of wild-type and TgH1aV.Fb mice. In wild-type mice, three TBP trains resulted in LTP in the paired pathway. TBP in slices from TgH1aV.Fb mice resulted in a transient potentiation of synaptic responses, lasting up to 30 minutes postpairing (red circles). TgH1aV.Fb mice completely lack the late component of TBP-induced potentiation found in wild-type mice (black squares). () The same protocol was applied to TgH1aV.Fb mice given dox in the drinking water from P21 until P42. Dox-treated mice (black traces), when compared to untreated ones (red traces), showed robust LTP (black squares), indicating rescue from sustained H1aV overexpression. () Fluorescent cells in hippocampal CA1 region 18 days after stereotaxic injection of lentivirus expressing either wild-type or mutant rat H1aV. LTP induced in hippocampal CA3-CA1 synapses of mice injected with lentivirus expressing H1aV () resulted in a transient potentiation of synaptic responses, whereas H1aV(W234A) expression showed normal LTP of the paired pathway (). Schematic drawing of the lentiviral vectors illustrates LTP profiles after α-CaMKII promoter-dependent transcription of wild-type () and mutant () H1aV. The amino acid substitution in the Homer1a EVH1 domain is indicated below the expression cassette for Homer1a.<p><b>Copyright information:</b></p><p>Taken from "Select Overexpression of Homer1a in Dorsal Hippocampus Impairs Spatial Working Memory"</p><p></p><p>Frontiers in Neuroscience 2007;1(1):97-110.</p><p>Published online Jan 2007</p><p>PMCID:PMC2518050.</p><p>