Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells-7

Ir. Cells were then placed in fresh media with or without 1 uM thapsigargin or 10 μg/ml tunicamycin for the time periods specified, followed by cell lysis and RNA purification. Spliced and unspliced XBP1 mRNA was detected using PCR methodology. Spliced XBP1 is 398 base pairs and the unspliced variant is 434 base pairs. For each lane the extent of splicing was quantified as described in the Methods section and is presented in additional file – .<p><b>Copyright information:</b></p><p>Taken from "Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells"</p><p>http://www.biomedcentral.com/1471-2407/8/229</p><p>BMC Cancer 2008;8():229-229.</p><p>Published online 11 Aug 2008</p><p>PMCID:PMC2527015.</p><p>