(A) Morphology of the MO– and MO–injected embryos at the 6-somite stage

The concentrantion for MO was 0.5 mM, and for each of MO, 0.3 mM. (B) Phenotype of the MO– and MO–injected pharyngula stage (24 hpf) embryos. The dorsalized phenotypes of the injected embryos were classified into four categories. 0, wild type; 1, modest enlargement of the telencephalon (arrowhead in panel 1); 2, strong anteriorization phenotype with enlargement of the head (arrowhead in panel 2) and reduction of the tail; and 3, very strong anteriorization phenotype with super enlargement of the telencephalon (arrowhead in panel 3) and major loss of trunk and tail. Bars, 500 μm. (C) Percentages of embryos were evaluated. Embryos were injected with MO or MO as indicated. The number of embryos scored () is shown on top of each bar. (D) Phenotype penetrance is shown. Embryos were coinjected with 0.5 mM MO or 0.2 mM MO2 with 20 pg/nl of . (E) morphants could be partially restored by 12 pg/nl of ΔN–β-catenin coinjection. Injection of the plasmids generates abnormal embryos with a reduced tail and small head (indicated as 4).<p><b>Copyright information:</b></p><p>Taken from "Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation"</p><p></p><p>The Journal of Cell Biology 2008;182(5):865-872.</p><p>Published online 8 Sep 2008</p><p>PMCID:PMC2528581.</p><p>