Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics-3

<p><b>Copyright information:</b></p><p>Taken from "Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics"</p><p>BMC Molecular Biology 2007;8():30-30.</p><p>Published online 10 May 2007</p><p>PMCID:PMC1884169.</p><p></p>c). DNA is represented as in Fig. 1 with recognition sites for III () and II () and PCR primers indicated. . Ethidium bromide stained agarose gel of electrophoresed PCR products generated with the primers O1 and O3 and cell pellets of 9 MPA/X, GFP-negative clones selected after transfection of Rht14-10 (Rht14-10IN) or of Rht14-19 (Rht14-19IN) with pIN-2 and pMC-Cre. M = marker DNA. Negative (-) and positive (+) controls used, respectively, no DNA and pTIGHTgpt DNA (expected product: 555 bp). . Southern blots of genomic DNA isolated from Rht14-10 and derivatives and digested with II (left), or from Rht14-19 and derivatives and digested with III (right). Derivatives before (10IN5 and 19IN3, 4 and 5) and after (10flp9.2 and 3 and 19flp9.2 and 12.3) Flp-mediated deletion are analysed. The probe was a fragment of the gene (methods)