Point mutations found in <i>wbiI</i> and <i>oacA</i> genes in clonal <i>B. pseudomallei</i> strains.

<p>These strains were collected chronologically from a single chronic lung patient who had severe bronchiectasis associated with melioidosis over almost 8 years. Panel A is the chronological order of these <i>B. pseudomallei</i> strains. Panel B demonstrates an extra base (“G”) that was found to cause frame-shift mutation in <i>wbiI</i> gene of all <i>B. pseudomallei</i> strains collected from day 550 onward. Panel C demonstrates the insertion of two extra bases “TC” in BPSL1936, the <i>oacA</i> homolog, in the same strains that had the <i>wbiI</i> mutation. Note: the <i>wbiI</i> gene of <i>B. pseudomallei</i> K96243 and <i>oacA</i> gene of <i>B. thailandensis</i> E264 <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001453#pntd.0001453-Brett1" target="_blank">[14]</a> were used as comparisons.