EMSA Analysis of SmMef2 DNA binding.

<p>Double stranded oligonucleotides with Mef2 consensus sequences were designed to test SmMef2 binding requirements (A). AT14 is the negative control probe (A). An MBP-SmMef2 fusion protein was purified and tested for its ability to bind double-stranded labeled probes (oligo-pair AT12 and oligo-pair AT13) containing different Mef2 consensus sequences (B and C, respectively). Purified MBP alone does not bind the DNA probes (B and C Lanes 2), while the MBP-SmMef2 fusion protein binds the probes (B and C, Lanes 3). Unlabeled oligonucleotide pairs AT11, AT12 and AT13 compete for binding against the labeled reporters (B and C, Lanes 4–6), while nonspecific AT14 does not compete (A and B Lane 7).