Cell lines UNS3-4A/A2-27.35 and UHCV/A2-27.

Benno Wölk (111163)
Claudia Trautwein (189767)
Benjamin Büchele (189771)
Nadine Kersting (189780)
Hubert E. Blum (6635)
Hans-Georg Rammensee (69883)
Andreas Cerny (189787)
Stefan Stevanovic (189791)
Darius Moradpour (15815)
Volker Brass (15814)

Publication date

January 2012

DOI

10.1371/journal.pone.0029286.g001

Abstract

<p>(A) Indirect immunofluorescence microscopy of UNS3-4A/A2-27.35 and UHCV/A2-27 cells cultured for 48 h in the presence (+ tet) or absence (− tet) of tetracycline. Monoclonal antibody 1B6 against HCV NS3 was used as primary antibody. (B) Immunoblot analysis of UNS3-4A/A2-27.35 and UHCV/A2-27 cells were cultured for 48 h in the presence (+ tet) or absence (− tet) of tetracycline. Monoclonal antibodies 1B6 against HCV NS3 or 11H against NS5A were used as primary antibodies. (C) Left panel: HLA-A2 surface expression of UNS3-4A/A2-27.35 (blue histogram) compared to the founder cell line UNS3-4A-24 (red histogram). Right panel: HLA-A2 surface expression of UHCV/A2-27 (blue histogram) compared to the founder cell line UHCVcon-57.3 (red histogram...