GR and GR-TR delay keratinocyte migration in <i>in vitro</i> wound scratch assays.

<p>A) <i>In vitro</i> migration of K5-GR and K5-GR-TR primary keratinocytes isolated from WT, K5-GR and K5-GR-TR were evaluated for <i>in vitro</i> wound closure at 8 h and 24 h following scratching. Phase contrast photographs are representative of four independent experiments. Bar: 100 µm. B) The graph shows the average of wound closure ± SD. Statistically significance was determined by ANOVA (*, p<0.05; **, p<0.01). The percentage of cell migration was estimated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029701#s4" target="_blank">Materials and Methods</a>. C) Quantitation of the p-ERK/ERK and p-AKT/AKT ratios in WT, K5-GR and K5-GR-TR keratinocytes before (t0) and after (t1) scratch wounding. Protein levels were determined by immunoblotting using specific antibodies against the total and phosphorylated proteins, and then normalized to actin. Mean values ± SD are shown. Asterisks denote statistically significant differences, as determined by ANOVA (*, p<0.05; **, p<0.005).