Cellular immune response 5 weeks post immunization.

<p>(a) Antigen-specific IFN-γ positive CD8⁺ T cells per million splenocytes, (b) Proportion responding CD8⁺ T cells as determined by intracellular staining of IFN-γ, IL-2 and TNF after SIINFEKL-peptide stimulation, or (c) proportion effector memory CD8⁺ T cells (pentamer H-2Kb/SIINFEKL positive CD8⁺CD62L⁻CD127⁺ cells) 5 weeks after the last intradermal immunization in combination with electroporation. Mice were either given one immunization (1×5 µg) or two immunizations 5 weeks apart (2×5 µg). Data shows average number of positive cells with error bars showing standard error of the mean. A booster immunization significantly increased the cellular memory response for both RREP-tLuc and DREP-tLuc (p<0.01). The total number of mice per group is indicated in parenthesis above each bar.