Pin1-null bone marrow-derived DC up-regulate costimulatory molecule expression in response to LPS.

<p>(A) Representative histograms showing expression of MHC class II (MHCII) and costimulatory molecules in WT and Pin1-null Mac1+ bone marrow-derived DC that were unstimulated or stimulated with 100 ng/ml LPS for 24 hours. The upper panel shows a histogram of CD11c expression in previously gated B220-Mac1+ cells. The gate shown in the histogram was applied to further analyze all CD11c+ cells for the expression of MHC class II (MHCII), CD40, and CD86 in the lower panels. Histograms represent data acquired from two independent experiments (n = 6). (B) Representative histograms showing expression costimulatory molecules in WT and Pin1-null Mac1− bone marrow-derived DC that were unstimulated or stimulated with 100 ng/ml LPS for 24 hours. The upper panel shows FACS plots of Mac1− cDC that were previously gated as B220-Mac1−. The gate in the upper panel (MHCII+CD11c+ gate) was applied to further analyze Mac1− cDC for the expression of CD40 and CD86 in the lower panels. Histograms represent data acquired from two independent experiments (n = 6). (C) Representative histograms showing expression of MHC class II (MHCII) and costimulatory molecules in WT and Pin1-null bone marrow-derived pDC that were unstimulated or stimulated with 100 ng/ml LPS for 24 hours. pDC were previously gated as PDCA1+CD11c+. Histograms represent data acquired from two independent experiments (n = 6).