Cell-cycle effects of GW8510 treatment.

<p>(A) FACS-generated histograms of propidium iodide stained cells treated with either vehicle control or GW8510 for 3 days. (B) Quantification of cell-cycle distributions from gated cellular populations in A expressed as percentage of the total cellular population. Data represent the mean ± SD of two biological replicates. (C) and (D) M-phase immunofluorescence analysis and quantification using histone H3 phospho-Ser10 as a mitosis marker. Total cells were counted using Hoechst nuclear stain. Representative images are shown for Hoechst, histone H3 phospho-Ser10, and overlay at indicated GW8510 concentrations. Values are expressed as fold over vehicle-treated controls. Data represent the mean ± SD of at least 3 biological replicates; ***p<0.001.