<p>Legend: (i) first set of experiment, with fibers activated at four Ca<sup>2+</sup> concentrations, (ii) second set of experiments, with fibers activated in presence or absence of blebbistatin, and (iii) third set of experiments, with fibers activated in pCa<sup>2+</sup> 4.5 (with and without blebbistatin) and pCa<sup>2+</sup> 6.0. The three sets of experiments correspond to the description given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029356#s2" target="_blank">Methods</a>.</p><p>*Significantly different from contractions produced at pCa<sup>2+</sup> 4.5 (p≤0.05).</p
<p>(A) In the experiments, the pressure imparted on the cell was (A-i) 50 hPa and (A-ii) 100 hPa. In...
<p>Normalized force (i.e., ratio of force at a given pCa and maximally activated force at pCa = 4.33...
(A) Maximal isometric force at pCa 4.6 (saturating [Ca2+]) was significantly smaller in the DOB grou...
<p>(A) Sample records from a typical experiment showing superimposed contractions produced by a fibr...
<p>(A) Superimposed contractions, showing the force decay during shortening while the fiber was acti...
<p>Sample records from a typical experiment showing the force produced by a muscle fiber activated i...
<p>(A) Superimposed contractions showing the force increase during stretch while the fibre was activ...
<p>Sample records from a typical experiment showing the force produced by a muscle fiber activated i...
<p>(A) Sample records recorded during a typical experiment in a fibre activated in pCa<sup>2+</sup>4...
<p>(A) Superimposed contractions showing the force decrease during shortening while the fiber was ac...
<p>(A) Superimposed contractions showing the force increase during stretch (top) when the fibre was ...
<p>Hill curves fit to force-pCa data for each individual fiber for <i>in vitro</i> data in <a href="...
<p>pCa<sub>50</sub>, pCa required for generation of 50% of maximal force. <i>n</i>, number of skinne...
<p>The myofilament Force-pCa curve shifts minimally to the right denoting the non-significant change...
<p>A) Examples of <i>k<sub>tr</sub></i> experiments on WT and NEB KO fiber bundles activated at pCa ...
<p>(A) In the experiments, the pressure imparted on the cell was (A-i) 50 hPa and (A-ii) 100 hPa. In...
<p>Normalized force (i.e., ratio of force at a given pCa and maximally activated force at pCa = 4.33...
(A) Maximal isometric force at pCa 4.6 (saturating [Ca2+]) was significantly smaller in the DOB grou...
<p>(A) Sample records from a typical experiment showing superimposed contractions produced by a fibr...
<p>(A) Superimposed contractions, showing the force decay during shortening while the fiber was acti...
<p>Sample records from a typical experiment showing the force produced by a muscle fiber activated i...
<p>(A) Superimposed contractions showing the force increase during stretch while the fibre was activ...
<p>Sample records from a typical experiment showing the force produced by a muscle fiber activated i...
<p>(A) Sample records recorded during a typical experiment in a fibre activated in pCa<sup>2+</sup>4...
<p>(A) Superimposed contractions showing the force decrease during shortening while the fiber was ac...
<p>(A) Superimposed contractions showing the force increase during stretch (top) when the fibre was ...
<p>Hill curves fit to force-pCa data for each individual fiber for <i>in vitro</i> data in <a href="...
<p>pCa<sub>50</sub>, pCa required for generation of 50% of maximal force. <i>n</i>, number of skinne...
<p>The myofilament Force-pCa curve shifts minimally to the right denoting the non-significant change...
<p>A) Examples of <i>k<sub>tr</sub></i> experiments on WT and NEB KO fiber bundles activated at pCa ...
<p>(A) In the experiments, the pressure imparted on the cell was (A-i) 50 hPa and (A-ii) 100 hPa. In...
<p>Normalized force (i.e., ratio of force at a given pCa and maximally activated force at pCa = 4.33...
(A) Maximal isometric force at pCa 4.6 (saturating [Ca2+]) was significantly smaller in the DOB grou...