Dynamic TC-core movements require HCV assembly.

<p>Huh-7.5 cells were electroporated with TC-core, bicistronic, or ΔNS2 RNAs. Electroporated cells were stained at 72 hours post electroporation with the biarsenical dye ReAsH and imaged using timelapse confocal microscopy where DsRed (200 ms) exposures were taken every 2 seconds. A. Timelapse montages of TC-core puncta transport of the viruses imaged. Time on x-axis. Elapsed times are 40sec (TC-core), 40sec (bicistronic), 132sec (ΔNS2) and height (distance traveled) of each montage is 12.48 µm B. Quantification of percent of TC-core puncta displaying movement over 1 µm during the course of imaging. Values are shown as a percentage of total cellular TC-core puncta. n-values equal total number of TC-core puncta, error bars are standard error (of the proportions). Asterisk indicates <i>p-value</i> <0.0001. C. Distance from origin plots of TC-core viruses. The cumulative distance traveled by a TC-core puncta was measured and plotted against time. n-values indicate total TC-core puncta tracked.