Transduction of Gsx2 changes the cell fate of postnatal OB progenitor cells <i>in vivo</i>.

<p>Retroviral particles expressing EGFP or Gsx2-EGFP were injected into the OB SEZ of P4 mice, and OB sections were obtained at P9 and immunostained with antibodies against GFP and Pax6 (A, B), Olig2 (C, D) or Dcx (E, F). Arrows and arrowheads indicate GFP⁺ cells expressing a particular cell marker, and the insets show high magnification images of double-labeled cells indicated with arrowheads in the main images. Gsx2 overexpression caused a 59.4% reduction (*P<0.05) in the number of Pax6⁺ expressing cells, a 110.4% increase (*P<0.05) in the number of Olig2⁺ cells, and a 33% decrease (*P<0.05) in the number of Dcx⁺ cells (G) when compared with controls. The graph in G represents the mean percentage ± s.e.m. of cells expressing GFP and Pax6 or Olig2 or Dcx (n = 3–4 animals per condition). Scale bar (shown in F) = 57.0 µm; insets = 23.4 µm.