Number of calls and vocal repertoire uttered in five behavioral contexts.

<p>A- Calls were recorded during social interaction task, novelty exploration, and restraint stress and analyzed off line. B- Total number of calls emitted in 4 minutes: SIT-isolated (n = 17), SIT-grouped (n = 8), EXPLO-isolated (n = 15), EXPLO-grouped (n = 8) and RESTRAINT (n = 16). C- Distribution and <b>s</b>pectrograms of the ten call types typically emitted by adult male mice. Data not shown, proportion of “other” calls: 1.4±0.8% in SIT-isolated, 4.5±1.3% in SIT-grouped, 9.5±4.1% in EXPLO-grouped, 3.1±1.3% in EXPLO-isolated and 16.3±3.1% in Restraint stress. (Time and frequency criterion were used to distinguish these categories, <i>see methods</i>). Data are presented as means ± SE. *: p<0.005; **: p<0.0001 for chi-square and Mann-Whitney paired comparisons.