Foxa1 up-regulates UCP1 and decreases MMP.

<p>Cultured cells were infected with Ad-Foxa1 or Ad-Control (48 h), the mRNA level of the mitochondrial uncoupling protein 1 (UCP1) was measured by real-time Q-RT-PCR (<b>A</b>), and the Mitochondrial Membrane Potential (MMP) was measured using the TMRM probe in an Olympus ScaR Screening Station (<b>B</b>). Data represent the mean±SD of 4–5 independent experiments. ** p<0.01; *** p<0.001. (<b>C</b>) Representative microphotographs of HepG2 cells and human cultured hepatocytes after 48 h of adenoviral transfection. Cells were stained with TMRM (red) for mitochondrial membrane potential and Hoechst 33342 (blue) for nuclei.