Macrophages from heat-treated mice produce more pro-inflammatory cytokines after <i>in vitro</i> re-stimulation.

<p><i>A</i> to <i>D</i>, BALB/c mice were injected with 10 µg of LPS and then received HT or were kept at RT for 2 hours. Peritoneal macrophages were isolated from these mice 2 hours post injection, recovered overnight and re-stimulated (2×10⁵/well) with LPS (100 ng/mL) and IFN-γ (25 U) <i>in vitro</i> at 37°C for 6 hours to determine TNF-α (<i>A</i>), IL-6 (<i>B</i>), and IL-10 (<i>D</i>) or 24 hours for IL-1β (<i>C</i>) production by ELISA. Cells from each treatment condition were pooled from 2 mice and measured in triplicate. Data are mean ± SD. Data are representative of three independent experiments. *<i>p</i><0.05; paired Student <i>t</i> test.