Generation of transgenic mice with cardiac specific over-expression of hPDCD5.

<p>(A), Schematic representation of the transgene construct of hPDCD5 cDNA under the control of the cardiac-specific alpha-MHC promoter (not at scale). (B), Representative western blot of hPDCD5 protein with PDCD5 antibody in heart extracts from high over-expressing line and WT control mice. Asterisk indicates endogenous PDCD5. (C), Densitometric analysis of PDCD5 immunoblots. (D), Representative western blot of hPDCD5 protein with HA antibody in heart extracts obtained from high over-expressing line and WT control mice. (E), Representative western blot of hPDCD5 protein with HA antibody in heart, liver, and brain extracts obtained from high over-expressing line and WT control mice.