Prediction of N-terminal coiled-coil structures in PARF-positive M proteins.

<p>(<i>A</i>) Coiled-coil structure prediction for the N-terminal sequences of the PARF-positive M proteins is given as P-scores vs. the amino acid position relative from the PARF motif with the first amino acid of the motif being position 1. The figure shows the superposition of all curves without indicating protein designations, however, collagen-binding M proteins are shown in <i>blue</i> and non-binding M proteins are shown in <i>red</i>. Single curves for all M proteins are provided as a supplementary figure (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030122#pone.0030122.s001" target="_blank">Figure S1</a>). The position of the PARF motif is highlighted in <i>light blue</i>. A <i>black dashed line</i> indicates the threshold value for coiled-coil prediction. Values below 0.025 indicate a coiled-coil structure. The prediction separates the M proteins into two classes; one class with (<i>B</i>) and one without (<i>C</i>) an N-terminal coiled-coil domain. They are shown in schematic representations that are not drawn to scale. <i>B</i> and <i>C</i> are not based on structural data other than coiled-coil prediction. The PARF motif is highlighted in <i>red</i>. The C-terminal end is labelled (-COOH).