Prediction of coiled-coil structures in PARF-positive M proteins.

<p>Coiled-coil structure prediction for the N-terminal sequences of the PARF-positive M proteins stG120.0 (<i>red dotted line</i>), stG120.1 (<i>light blue solid line</i>) and stGM220 (<i>black dotted line</i>) is given as P-scores vs. the amino acid position relative from the PARF motif with the first amino acid of the motif being position 1. Isolates with M protein stG120.1 bind collagen IV, while strains that carry one of the other two M proteins do not. The position of the PARF motif is highlighted in <i>light blue</i>. A <i>black dashed line</i> indicates the threshold value for coiled-coil prediction. Values below 0.025 indicate a coiled-coil structure.