Use of plasmid pKS5K to cure the megaplasmid pXO1 from wild type strain A16.
- Xiankai Liu (188445)
- Dongshu Wang (188449)
- Huagui Wang (188452)
- Erling Feng (188457)
- Li Zhu (67404)
- Hengliang Wang (188464)
Publication date
January 2012
DOIAbstract
<p>(A) Results of PCR analysis show the loss of anthrax toxin genes <i>pagA</i>, <i>lef</i>, and <i>cya</i> from pXO1-cured strain A16Q1. (B) Capsule formation was observed in both wild type strain A16 and pXO1-cured strain A16Q1. (C) Results of western blot analysis show the expression of anthrax toxin protective antigen protein in pA16, but not in A16Q1.</p
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