Localization of GAP activity-defective Bfa1 mutants.

<p>(A) The anaphase localization of Bfa1 mutants. <i>cdc15-2SPC42-RFP</i> cells expressing the indicated <i>BFA1-GFP</i> mutants (YSK2545, 2547, 2549, 2551, 2553, and 2557) were synchronized with α-factor at 25°C, released at 35°C for 3 h, and processed for confocal microscopy. (B) The quantification of (A). The average of two independent counts is plotted with standard deviations (n = 200). Arrows indicate Bfa1 localized to the SPB^m. Bar, 5 µm. (C) <i>In vitro</i> GTPase assays of wild-type MBP-Bfa1 and MBP-Bfa1^DDR2 (5 µg), as described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002450#s4" target="_blank">Materials and Methods</a>.